Cell viability was determined by using the CellTiter-Glo kit (Promega) as described previously (37 (link), 59 (link)). Briefly, HEK293T cells seeded in opaque-walled 96-well plates were transfected with siRNA targeting ANP32A or with AllStars negative-control siRNA at a concentration of 30 nM. At 48 h posttransfection, luminescence was measured with the CellTiter-Glo kit (Promega).
The cell viability assay was similarly performed for the Anp32a-KO HEK293T cells, Anp32a-KO A549 cells, and the corresponding control cells grown in opaque-walled 96-well plates.
The cell viability assay was similarly performed for the Anp32a-KO HEK293T cells, Anp32a-KO A549 cells, and the corresponding control cells grown in opaque-walled 96-well plates.
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