MALDI Imaging of Brain and Liver Tissues

One image of a sagittal brain tissue section and six liver tissue sections (three slices from a control animal and three sections from a THS-exposed animal) were acquired using a MALDI TOF/TOF UltrafleXtreme instrument with SmartBeam II Nd:YAG/355 nm laser from Bruker Daltonics, also at the COS facilities. Raster sizes of 80 and 20 µm were used for the brain and liver tissue sections, respectively. The TOF spectrometer operated in reflectron positive mode with the digitizer set at a sample rate of 1.25 GHz in a mass range between 70 and 1200 Da. The spectrometer was calibrated prior to tissue image acquisitions using [Au]⁺ cluster MS peaks as internal mass references [27 (link)].