Molecular Mechanisms of NEAT1 Regulation

Transfection was conducted via Lipofectamine 2000 in accordance to the manufacturer’s specifications. A NEAT1 overexpression plasmid, pcDNA3.1-NEAT1, was commercially constructed by Genechem Co., Ltd. (Shanghai, China). The NEAT1 siRNA sequences used were 5′-GUGAGAAGUUGCUUAGAAACUUU-3′ and 5′-GGAAAGUUUCUAAGCAACUUCUCAC-3′. The siRNA for Bcl-2 was 5′-AACAUCGCCCUGUGGAUGACU-3′. The control counterparts were 5′-GUACCUGACUAGUCGCAGAAG-3′ and 5′-UCUGCGACUAGUCAGGUACGG-3′. All sequences were synthesized and bought from GenePharma. On the other hand, the miR-129-5p mimic, miR-129-5p antagonist, and controls were purchased from RiboBio [38 (link)].