Protocol detail

Gut Microbiome Sampling Protocol

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Fecal samples were collected between 2016 and 2017 from 13 healthy men and women (UCC employees and students, residents of Cork, Ireland), in accordance with study protocol APC055³³ (link) approved by the Cork Research Ethics Committee. Samples were anonymized by APC Microbiome Ireland research nurses and issued with unique subject-specific identifiers (Table S1). One of the subjects (916) was sampled twice. More extensive participant metadata is available from the original cohort study publication.³³ (link) Samples were collected in participants homes and delivered to the lab in tightly closed containers within 2–3 h after voiding. Immediately upon delivery samples were aseptically aliquoted into 1 g portions (in triplicates) and placed into individual Sterilin containers. Samples were then incubated anaerobically with Anaerocult A pouches in anaerobic jars (Merck Millipore) at 37°C and frozen at −80C at the indicated time points.

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Shkoporov A.N., O'Regan O., Smith L., Khokhlova E.V., Draper L.A., Ross R.P, & Hill C. (2023). Dynamic nature of viral and bacterial communities in human faeces. iScience, 27(2), 108778.

Publication 2023

Anaerocult A pouches anaerobic jars

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Healthy men and women (UCC employees and students, residents of Cork, Ireland)
Fecal samples collected between 2016 and 2017
Samples anonymized and issued with unique subject-specific identifiers
Samples collected in participants' homes and delivered to the lab within 2-3 hours after voiding
Samples aseptically aliquoted into 1 g portions (in triplicates) and placed into individual Sterilin containers
Samples incubated anaerobically with Anaerocult A pouches in anaerobic jars at 37°C and frozen at -80°C

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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