Five microliters of miR-44438–NCMB was applied to a copper grid with a carbon support membrane. After a 60-s incubation, the excess sample was removed with filter paper and stained with a 2% uranyl acetate solution for 60 s. The excess uranyl acetate solution was removed with filter paper and by air-drying. Electron microscope images were collected using a Tecnai F20 TEM at 200 kV.
Protocol detail
Visualizing miR-44438–NCMB using TEM
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Corresponding Organization : Capital Medical University
Other organizations : Yidu Central Hospital of Weifang, Tianjin Huanhu Hospital, Air Force Medical University, Aerospace Center Hospital, Beijing Tian Tan Hospital, Xuzhou Medical College, Union Hospital, Jilin University, UNSW Sydney
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Variable analysis
independent variables
- Volume of miR-44438–NCMB applied to the copper grid (5 microliters)
- Electron microscope images of the sample
- Incubation time of the sample on the copper grid (60 s)
- Staining time with 2% uranyl acetate solution (60 s)
- Electron microscope used (Tecnai F20 TEM)
- Accelerating voltage of the electron microscope (200 kV)
- Carbon support membrane on the copper grid
- No positive or negative controls were explicitly mentioned in the input protocol.
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