Sequencing and Methylation of C. thermocellum

Genomic DNA from C. thermocellum ATCC 27405 was isolated using the Genomic Tip kit (Qiagen according to the manufacturer’s instructions and sent to Expression Analysis (Durham, NC, USA) for sequencing on a Pacific Biosciences (PacBio) instrument. Single-molecule real-time (SMRT) sequencing was performed using the PacBio RS technology with four SMRT cells. Methylated sequences were determined by Expression Analysis using the SMRT Analysis software [10 (link)].

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Riley L.A., Ji L., Schmitz R.J., Westpheling J, & Guss A.M. (2019). Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction–modification systems. Journal of Industrial Microbiology & Biotechnology, 46(9), 1435-1443.

Publication 2019

Genomic Tip kit

Cells Genomic Single molecule analysis

Corresponding Organization : Oak Ridge National Laboratory

Other organizations : University of Georgia

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Genomic DNA from C. thermocellum ATCC 27405
Genomic Tip kit (Qiagen) used according to manufacturer's instructions
SMRT sequencing performed using PacBio RS technology with four SMRT cells
Methylated sequences determined by Expression Analysis using SMRT Analysis software

controls

Positive control: Not mentioned
Negative control: Not mentioned

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