As described in our previous study (25 (link)), EpiQuik™ Tissue ChIP Kit (P-2012; Epigentek Group Inc., Farmingdale, NY, USA) was used to perform the ChIP assay according to the manufacturer's protocol. In brief, the acetylated histone H3 antibody (1 µg, Epigentek Group Inc.) or 1 µl of normal mouse IgG (as a negative control) was used to pre-coat the assay wells. Meanwhile, 30 mg of heart tissue was cut into little pieces and cross-linked with 1% formaldehyde. The cross-link was stopped by glycine solution (1.25 M). After tissue disaggregation and the nuclei isolation, the DNA was sheared by sonication (S-450-Dwithmicro-tip probe; Emerson Industrial, St. Louis, MO, USA) with 5 pulses of 20 s each separated by 40 s rest on ice (output control: 2). After centrifugation, 5 µl of the diluted supernatants were used as input DNA. The other diluted supernatant (100 µl) was added to the acetylated histone H3 antibody-coated wells followed by incubation at room temperature for 60 min. ChIP-enriched DNA fragments were precipitated, purified, and assayed by quantitative PCR with the following primers: DUSP5 forward 5’ CTGACACTCCACCGGTAGTC 3’, reverse 5’ GGGCGTCTTAGAGCGAGAAA 3’. The value was computed relative to a calibrator (2−ΔΔCt) and normalized to the input.