Polymer-Based Delivery Systems for Immunotherapy

α-galactosylceramide
(α-Galcer) was purchased from Cayman Chemical. Poly(D, L- lactide-co-glycolide
(a mole ratio of 50:50, RESOMER RG 502 H, PLGA), Ovalbumin, Sephadex
G50, Dulbecco’s phosphate buffered saline (PBS), dialysis kit
Pur-A-Lyzer Maxi-6000 MWCO 6–8 kDa, and Amicon Ultra-4 Centrifugal
filter unit were all purchased from Sigma-Aldrich. Ovalbumin Alexa-Fluor
488 and Ovalbumin Alexa-Fluor 647 were purchased from Thermo Fischer
Scientific. Human IFN-γ uncoated ELISA and Mouse IL-2 Uncoated
ELISA kits were purchased from Invitrogen. Solvents were purchased
from Lachner and dried over molecular sieves (3 Å). The block
copolymers poly[N-(2-hydroxypropyl)methacrylamide]-b-poly[N-(4-isopropylphenylacetamide)ethyl
methacrylate] (PHPMA₂₅-b-NR₃₃, herein named NR block), poly[N-(2-hydroxypropyl)methacrylamide]-b-poly[N-(4-ethylamino]carbonyloxymethyl)
phenylboronic acid pinacol ester methacrylate] (PHPMA₂₅-b-MRE₃₀, herein named MRE block), and
poly[N-(2-hydroxypropyl)methacrylamide]-b-poly[N-(4-isopropylamino]carbonyloxymethyl) phenylboronic
acid pinacol ester methacrylate] (PHPMA₂₅-b-MRI₂₆, herein named MRI block) were synthesized as previously
described.^{23 (link)} The block copolymer poly[N-(2-hydroxypropyl)methacrylamide]-b-poly[4-(4,4,5,5-tetra-methyl-1,3,2-dioxaborolan-2-yl)benzyl
methacrylate] (PHPMA₃₇-b-ROS₄₂, herein named ROS block) and the block copolymer poly([N-(2-hydroxypropyl)] methacrylamide)-b-poly[2-(diisopropylamino)ethyl
methacrylate] (PHPMA₃₅-b-PDPA₇₅, herein named pH block) were synthesized according to our previously
reported synthetic pathways.^{24 (link),25 (link)} The subscripts refer
to the degrees of polymerization of each block, as determined by ¹H NMR. Table S1 reveals the polymer
block physicochemical characteristics.