Western Blot Protein Expression Analysis
Corresponding Organization : University of Würzburg
Other organizations : Tel Aviv University, Whitehead Institute for Biomedical Research, Robert Bosch Hospital, Weizmann Institute of Science
Variable analysis
- Equal amounts of protein (typically 30–40 μg)
- Protein expression analyzed by Western blot
- SDS-PAGE separation
- Blotting onto nitrocellulose membrane (0.1 μm; GE Healthcare) by semi-dry blotting (1 mA/cm^2, 1 h)
- Quantitative densiometric analyses from Western blots conducted with imageJ
- None explicitly mentioned
Annotations
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