Protein expression was analyzed by Western blot as described elsewhere [50 (link)]. Equal amounts of protein (typically 30–40 μg) were separated by SDS-PAGE and blotted (Biometra FastblotTM, Analytic Jena) onto nitrocellulose membrane (0.1 μm; GE Healthcare) by semi-dry blotting (1 mA/cm2, 1 h). Quantitative densiometric analyses from Western blots were conducted with imageJ.
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