Ants were manually collected from 35 nests throughout northeastern Argentina. Identification was done up to morphospecies, since updated keys for Nylanderia species are not available for the studied region, resulting in: 30, 2, 2 and 1 samples of N. fulva, Nylanderia sp. 1, Nylanderia sp. 2 and P. longicornis, respectively (Table S1). Genomic DNA was extracted from a single worker ant per nest using Extract-N-Amp Tissue PCR kit (Sigma-Aldrich Inc., St. Louis, MO, USA). Ant cytochrome C oxidase subunit I (COI) gene fragments were amplified by PCR using Jerry and Pat primer pair (18 (link)) and sequencing was performed by Macrogen (Macrogen Inc., Seoul, South Korea).
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