Sensitive NGF Methodology for Plasma Cell Detection
Follow-up samples were processed within 24 h after collection. Analysis was carried out using the recently developed NGF methodology (Table S1), following the EuroFlow guidelines, as described elsewere20 (link),22 (link). Events from two eight-color tubes (per sample) were merged using the merge function of the INFINICYT™ v2.0 software (Cytognos S.L. Salamanca, Spain). A sample was considered positive when at least 20 aberrant plasma cells were detected. Hemodilution of bone marrow samples for NGF evaluation was assessed through the identification of a significant decrease in non-plasma cell populations: mast cells (CD117hi), erythroblasts (CD45−/sideward-scatterlo) and B-cell precursors (CD19+/CD38hi/CD45lo). The complete analysis of NGF performance for the GEM2012 trial has already been published28 (link).
Other organizations :
Instituto de Investigación Biomédica de Salamanca, Centro de Investigación Biomédica en Red de Cáncer, Centro de Investigación del Cáncer, Institut Català d'Oncologia, Consorci Institut D'Investigacions Biomediques August Pi I Sunyer, Vall d'Hebron Hospital Universitari, Hospital Universitario de Canarias, Research Institute Hospital 12 de Octubre, Clinica Universidad de Navarra
Detection of at least 20 aberrant plasma cells in a sample
control variables
Hemodilution of bone marrow samples for NGF evaluation was assessed through the identification of a significant decrease in non-plasma cell populations: mast cells (CD117hi), erythroblasts (CD45−/sideward-scatter-lo) and B-cell precursors (CD19+/CD38hi/CD45lo)
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