Culture supernatants were harvested and centrifuged to remove cellular debris, and aliquots were stored at –80° C until assayed. The concentrations of IL-10, Arg1, iNOS, and TNF-α were determined using corresponding human ELISA kits (IL-10: D1000B, R&D Systems; Arg1: P05089, Cusabio, Houston, TX, USA; iNOS: P35228, Cusabio; TNF-α: DTA00D, R&D Systems) following the methods described previously [62 (link)].
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