For osteogenic differentiation, after 60% confluency was reached, the hBMSCs were cultured in an osteogenic induction medium that contained complete medium supplemented with 50 μg/mL ascorbic acid (Solarbio, Beijing, China), and 10 mmol/L β-glycerophosphate (Solarbio, Beijing, China), with Dex (10−8, 10−7, and 10−6 mol/L) for 14 days [19 (link)]. The osteogenic induction medium was replaced every 2 days. The hBMSCs were treated with the solvent of Dex as the control.
For adipogenic differentiation, after 80% confluency was reached, the hBMSCs were cultured in an adipogenic induction medium that contained complete medium supplemented with 500 μmol/L isobutylmethylxanthine (Solarbio, Beijing, China), 100 μmol/L indomethacin (Solarbio, Beijing, China), and 10 μg/mL insulin (Solarbio, Beijing, China), with or without Dex (10−8, 10−7, 10−6 mol/L) for 4 days. Then, the adipogenic induction medium was changed to a maintenance medium that contained the complete medium supplemented with 10 μg/mL insulin and 5 μmol/L pioglitazone (Solarbio, Beijing, China), with or without Dex (10−8, 10−7, and 10−6 mol/L) for 10 days [19 (link)]. The medium was replaced every 2 days. The hBMSCs were treated with the solvent of Dex as the control.
For adipogenic differentiation, after 80% confluency was reached, the hBMSCs were cultured in an adipogenic induction medium that contained complete medium supplemented with 500 μmol/L isobutylmethylxanthine (Solarbio, Beijing, China), 100 μmol/L indomethacin (Solarbio, Beijing, China), and 10 μg/mL insulin (Solarbio, Beijing, China), with or without Dex (10−8, 10−7, 10−6 mol/L) for 4 days. Then, the adipogenic induction medium was changed to a maintenance medium that contained the complete medium supplemented with 10 μg/mL insulin and 5 μmol/L pioglitazone (Solarbio, Beijing, China), with or without Dex (10−8, 10−7, and 10−6 mol/L) for 10 days [19 (link)]. The medium was replaced every 2 days. The hBMSCs were treated with the solvent of Dex as the control.
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