Proteasomal Chymotrypsin-like Activity Assay
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Corresponding Organization :
Other organizations : Guangzhou Medical University, The Barbara Ann Karmanos Cancer Institute, Wayne State University, University of South Dakota
Protocol cited in 5 other protocols
Variable analysis
- Shikonin at different concentrations
- Hydrolysis of the fluorogenic substrates (Suc-LLVY-AMC)
- Purified rabbit 20S proteasome (35 ng)
- Suc-LLVY-AMC (40 μmol/L)
- Assay buffer (20 mM Tris-HCl, pH 7.5)
- Incubation time (2 h)
- Temperature (37°C)
- DMSO (solvent control)
- Positive control: Purified rabbit 20S proteasome incubated with Suc-LLVY-AMC in the absence of shikonin (DMSO control)
- Negative control: Not explicitly mentioned
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