Protocol detail

Comprehensive Liquid Biopsy Analysis

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cfDNA testing was performed during routine standard-of-care treatment using a commercially available panel, Guardant360 (Guardant Health, Inc., Redwood City, CA, USA) [16 (link)] and FoundationOne Liquid CDx (Foundation Medicine, Inc., Cambridge, MA, USA) [17 (link)]. Tumor sequencing was done using FoundationOne CDx (Foundation Medicine, Inc., Cambridge, MA, USA) and Tempus xT (Tempus, Inc., Chicago, IL, USA). All variant types were analyzed, including small mutations, copy number alterations, and gene rearrangements. Variants of unknown significance were excluded.

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McFarland T.R., Mathew Thomas V., Nussenzveig R., Gebrael G., Sayegh N., Tripathi N., Sahu K.K., Goel D., Maughan B.L., Sirohi D., Agarwal N, & Swami U. (2022). Detection of BRCA1, and BRCA2 Alterations in Matched Tumor Tissue and Circulating Cell-Free DNA in Patients with Prostate Cancer in a Real-World Setting. Biomedicines, 10(12), 3170.

Publication 2022

FoundationOne Liquid CDx FoundationOne CDx

Cfdna Copy number alterations Gene rearrangements Medicine Mutations Redwood Tumor

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Variable analysis

dependent variables

Tumor sequencing results, including small mutations, copy number alterations, and gene rearrangements

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