Tissue Biopsy RNA Extraction Protocol

Immediately after collection, wound tissue biopsies were rinsed in saline, patted dry and snap frozen in liquid nitrogen. Grinding of the tissues was performed using a 6770 Freezer/Mill® cryogenic grinder (SPEX SamplePrep, Metuchen, NJ). Total RNA from tissue or cultured cells were extracted using mirVana RNA isolation kit (Ambion, Austin, TX) as described (5 (link), 13 (link)).

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Elgharably H., Ganesh K., Dickerson J., Khanna S., Abas M., Ghatak P.D., Dixit S., Bergdall V., Roy S, & Sen C.K. (2015). A Modified Collagen Gel Dressing Promotes Angiogenesis in a Pre-Clinical Swine Model of Chronic Ischemic Wounds. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 22(6), 720-729.

Publication 2014

6770 Freezer/Mill® mirVana RNA isolation kit

Austin Cultured cells Frozen Isolation Nitrogen Saline Tissue Wound biopsies

Corresponding Organization : The Ohio State University

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Variable analysis

independent variables

Grinding of the tissues using a 6770 Freezer/Mill® cryogenic grinder (SPEX SamplePrep, Metuchen, NJ)

dependent variables

Total RNA from tissue or cultured cells

control variables

Wound tissue biopsies were rinsed in saline, patted dry and snap frozen in liquid nitrogen prior to grinding

controls

No positive or negative controls were explicitly mentioned.

Annotations

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