As previously described by Voorham et al[12 (link)], the paraffin-embedded sections were incubated for five days in ATL lysis buffer (QIAmp DNA Micro Kit, Qiagen, Beijing, China). Proteinase K (10 μL from a 20 ng/μL solution) was added daily. DNA isolation was performed using a column-based technique (QIAmp DNA Micro Kit, Qiagen). A Beckman UV-vis spectrophotometer (Beckman, CA, United States) was used to measure DNA concentrations and purities.
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DNA Extraction from Paraffin-Embedded Tissues
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Other organizations : Chongqing Medical University
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Variable analysis
independent variables
- Incubation duration (5 days)
- DNA concentration
- DNA purity
- Paraffin-embedded sections
- ATL lysis buffer (QIAmp DNA Micro Kit, Qiagen, Beijing, China)
- Proteinase K (10 μL from a 20 ng/μL solution)
- DNA isolation using column-based technique (QIAmp DNA Micro Kit, Qiagen)
- Beckman UV-vis spectrophotometer (Beckman, CA, United States)
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