Immunoblotting with anti-SRSF2 antibody was performed as previously described (34 (link)). Briefly, cells were harvested and lysed with lysis buffer for 30 min at 4°C. The supernatants were collected and separated on 12% SDS-PAGE, and then transferred to PDVF membrane. After blocking with 5% skim milk, membrane was incubated anti-SRSF2 (Millipore) or anti-U2AF65 antibodies. The signal was detected by enhanced chemiluminescence (ECL).
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Immunoblotting of SRSF2 in Cells
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Other organizations : Gwangju Institute of Science and Technology, Nantong University, Korea Research Institute of Bioscience and Biotechnology, Konkuk University
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Variable analysis
independent variables
- Immunoblotting with anti-SRSF2 antibody
- Signal detected by enhanced chemiluminescence (ECL)
- Cells were harvested and lysed with lysis buffer for 30 min at 4°C
- Supernatants were collected and separated on 12% SDS-PAGE, and then transferred to PDVF membrane
- Membrane was incubated anti-SRSF2 (Millipore) or anti-U2AF65 antibodies after blocking with 5% skim milk
- Anti-U2AF65 antibody as a positive control
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