Standardized Biomarker Measurements for Epidemiological Studies

The NHS and NHSII heparinized plasma samples were assayed for total cholesterol, HDL-C, direct LDL-C, and triglycerides using an ARCHITECT ci8200 analyzer (Abbott Diagnostics, Chicago IL). High-sensitivity C-reactive protein (CRP) levels were measured via a validated immunoturbidometric method (Denka Seiken, Tokyo, Japan) (20 (link)). Matched case-control sets were assayed together in the same batch. Coefficients of variation (CVs) calculated from blinded replicate samples randomly interspersed with participant samples were ≤2% across all assay batches. These biomarkers are stable within person over time, with intraclass correlations over 1 year of 0.60–0.84 (21 (link)). In the UK Biobank, assays were performed at a dedicated central laboratory between 2014 and 2017, including measurement of serum total cholesterol, HDL-C, direct LDL-C, and triglycerides (Beckman Coulter AU5800). High-sensitivity CRP was measured by immunoturbidimetric analysis (Beckman Coulter AU5800). CVs for the UK Biobank assays were ≤3% across all assay batches (https://biobank.ndph.ox.ac.uk/showcase/showcase/docs/serum_biochemistry.pdf).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Trabert B., Hathaway C.A., Rice M.S., Rimm E.B., Sluss P.M., Terry K.L., Zeleznik O.A, & Tworoger S.S. (2021). Ovarian cancer risk in relation to blood cholesterol and triglycerides. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 30(11), 2044-2051.

Publication 2021

ARCHITECT ci8200 analyzer AU5800

Assay Biomarkers Cholesterol Diagnostics High sensitivity c reactive protein Immunoturbidimetric Plasma Replicate Serum Triglycerides

Corresponding Organization : Huntsman Cancer Institute

Other organizations : Moffitt Cancer Center, Massachusetts General Hospital, Harvard University, Brigham and Women's Hospital

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total cholesterol
HDL-C
Direct LDL-C
Triglycerides
High-sensitivity C-reactive protein (CRP) levels

control variables

Matched case-control sets were assayed together in the same batch
Coefficients of variation (CVs) calculated from blinded replicate samples randomly interspersed with participant samples were ≤2% across all assay batches
Biomarkers are stable within person over time, with intraclass correlations over 1 year of 0.60–0.84

controls

Blinded replicate samples randomly interspersed with participant samples

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!