The aqueous humor and vitreous humor were used as collected. The retina choroid and iris-ciliary bodies used in this study were homogenized in ice-cold IPBS in an ice bath using a TISSUEMISER (Fisher Scientific). The homogenates were then centrifuged at 17,000g at 4°C for 15 minutes. The protein contents of the supernatants were determined according to the method of Bradford34 (link) and were adjusted to approximately 1 mg/mL.
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Tissue Homogenization and Protein Extraction
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Corresponding Organization : University of Mississippi
Other organizations : ElSohly Laboratories (United States)
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Variable analysis
independent variables
- None explicitly mentioned
- Protein contents of the supernatants
- The aqueous humor and vitreous humor were used as collected
- The retina choroid and iris-ciliary bodies were homogenized in ice-cold IPBS in an ice bath using a TISSUEMISER (Fisher Scientific)
- The homogenates were then centrifuged at 17,000g at 4°C for 15 minutes
- The protein contents of the supernatants were determined according to the method of Bradford and were adjusted to approximately 1 mg/mL
- No positive or negative controls were explicitly mentioned
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