Murine Models for Immunology Research

C57BL/6J WT, BALB/c WT, TLR4 mutant (C3HeJ), Tlr4^−/−, Il18r^−/−, Il12p40^−/−, Tlr2^−/−, Tlr5^−/−, Il1r1^−/−, Il12p35^−/−, Langerin-eGFP-DTR, Cd11c-Cre, and B6.SJL-Ptprc^aPep3^b/BoyJ (OT-II) transgenic mice were purchased from the Jackson Laboratory. C3HeB/FeJ mice were purchased from Taconic. DO11.10 TCR transgenic mice were purchased from Charles River Laboratory. Myd88^−/−, Trif^−/− (Hise et al., 2007 (link)), Il22^−/− (Zheng et al., 2007 (link)), IIl23r^−/− (Chan et al., 2006 (link)), Unc93b1^−/− (Kim et al., 2008 (link); provided by H. Ploegh, Whitehead Institute for Biomedical Research, Cambridge, MA), Il23p19^−/− (Edgerton et al., 2009 (link); provided by M. Oukka, Center of Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, WA), and Il1rl1^−/− mice (Barlow et al., 2013 (link)) were previously described. All mice were kept in a pathogen-free environment and fed an OVA-free diet. GF mice were bred at the gnotobiotic Research Animal Facility at The University of Chicago. All procedures performed on the mice were in accordance with the Animal Care and Use Committee of the Children's Hospital Boston.