The patient’s clinicopathological characteristics were summarized descriptively and tested using two-sample Student t-tests (continuous variables) and Pearson’s Chi-square tests (categorical variables). Wilcoxon–Mann–Whitney tests were used to analyze the TNM stage and histological grade. The DFS and DSS of subgroups were compared to determine survival outcomes using Kaplan–Meier curves and then further analyzed with the log-rank test to observe the significance. All possible prognostic factors were determined using a univariate Cox proportional hazards model; subsequently, the meaningful prognostic factors (defined as those with P<0.05 in univariate analysis) were further assessed in a multivariate analysis. Interaction between EYA2 expression status and chemotherapy was evaluated using Cox proportional hazards models with a 2×2 factorial design.21 (link) All statistical analyses were conducted using SPSS V.19.0 for Windows (IBM Corporation, Armonk, NY, USA). Significance was set at P<0.05 for two-sided tests.