Peripheral blood samples were frozen at −80°C. All samples from each experiment were thawed and analyzed for dasatinib concentration at the same time. Once thawed, dasatinib concentrations were determined using an assay developed to monitor a structurally similar compound IM by LC/LC-MS/MS (27 (link)), with minor modifications. Using this assay, concentrations of dasatinib in whole blood can be determined with a lower limit of detection of 50pg/ml. PK parameters such as half-life (T½), peak concentration (Cmax), and area under the curve (AUCinf) were calculated using WinNonlin software (Pharsight, St. Louis, MO).
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Dasatinib Pharmacokinetics Analysis
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Corresponding Organization : University of Colorado Denver
Other organizations : National Cancer Institute
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Variable analysis
independent variables
- None explicitly mentioned
- Dasatinib concentration in whole blood
- All samples from each experiment were thawed and analyzed for dasatinib concentration at the same time
- Dasatinib concentrations were determined using an assay developed to monitor a structurally similar compound IM by LC/LC-MS/MS, with minor modifications
- PK parameters such as half-life (T1/2), peak concentration (Cmax), and area under the curve (AUCinf) were calculated using WinNonlin software
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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