Luminex-based Neurodegenerative Disease Panel

Luminex kits for human neurodegenerative disease panel three (PDGF-AA and PDGF-AB/BB) were acquired from Millipore (cat. no. HNDG3MAG-36K) (Millipore Inc Billerica, MA, USA) and performed according to manufacturer instructions. Pre-treatment of serum samples and parameters in a FLEXMAP3D (Luminex, TX, USA) were similar to previous reports.18 (link) Median fluorescence intensity (MFI) was determined and used to estimate serum concentration of PDGF-AA and PDGF-AB/BB. Protein concentrations were calculated according to the standard curve using a known concentration.

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Ma R., Yang Q., Cao S., Liu S., Cao H., Xu H., Wu J, & Feng J. (2020). Serum Platelet-Derived Growth Factor Is Significantly Lower in Patients with Lung Cancer and Continued to Decrease After Platinum-Based Chemotherapy. OncoTargets and therapy, 13, 1883-1892.

Publication 2020

PDGF-AA PDGF-AB/BB HNDG3MAG-36K FLEXMAP3D

Fluorescence Human Neurodegenerative disease Pdgf aa Pdgf bb Protein Samples treatment Serum

Corresponding Organization :

Other organizations : Jiangsu Cancer Hospital, Nanjing Medical University, Jiangsu University, Xuzhou Cancer Hospital, Nanjing Tech University

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Variable analysis

independent variables

Pre-treatment of serum samples

dependent variables

Median fluorescence intensity (MFI)
Serum concentration of PDGF-AA and PDGF-AB/BB

control variables

Parameters in a FLEXMAP3D (Luminex, TX, USA)
Similar to previous reports

controls

Standard curve using a known concentration

Annotations

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