The following antibodies were used for IFA and Western blot: rabbit polyclonal anti-HA (Life Technologies) (IFA: 1:100); rabbit polyclonal anti-HA (Sigma) (WB 1:1000); rat anti-HA mAb clone 3F10 (Roche) (IFA: 1:100, WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:100, WB 1:500); mouse anti-EXP2 mAb clone 7.733 (link) (IFA: 1:1000, WB: 1:1000); mouse anti-HRP2 mAb 2G1214 (link) (IFA 1:1000, WB: 1:500); mouse anti-RESA mAb clone 28/234 (IFA: 1:1000, WB: 1:1000); rabbit polyclonal anti-BiP35 (link) (WB: 1:1000); rabbit polyclonal anti-REX136 (link) (IFA: 1:1000); mouse polyclonal anti-REX215 (link) (IFA: 1:500); mouse polyclonal anti-REX315 (link) (IFA: 1:500); rabbit polyclonal anti-SBP121 (link) (IFA: 1:500); mouse polyclonal anti-MSRP622 (link) (IFA: 1:500); mouse polyclonal anti-PfGECO27 (link) (IFA: 1:300); rabbit polyclonal anti-Pfs1637 (link) (IFA: 1:500); rabbit polyclonal anti-PfEMP1 ATS38 (link) (IFA: 1:200); mouse polyclonal anti-CLAG328 (link) (IFA: 1:300); rabbit polyclonal anti-PM2 antibody 73739 (link) (WB 1:2000); rabbit polyclonal anti-KAHRP16 (link) (IFA: 1:500); rabbit polyclonal anti-SERP40 (link) (IFA: 1:500, WB: 1:1000).
Protocol detail
Comprehensive Antibody Characterization for Malaria Research
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Corresponding Organization :
Other organizations : Washington University in St. Louis
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Variable analysis
independent variables
- Antibodies used for IFA and Western blot
- Localization and expression of target proteins
- Concentration of antibodies used (1:100, 1:1000, 1:500, etc.)
- Positive control: None mentioned
- Negative control: None mentioned
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