C. elegans Strains and Antibodies

C. elegans strains were cultured as previously described by Brenner [11 (link)]. Strain VS20 hjIs67[Patgl-1::atgl-1::GFP] [12 (link)] was obtained from CGC and subsequently crossed into CB1370 daf-2(e1370) and MR1000 daf-2(e1370); aak-1(tm1944); aak-2(ok524) strains. Strains MR1348 daf-2(e1370); rrEx226[sur-5p::GFP::ATGL-1::HA; rol-6(gf)] and MR1413 daf-2(e1370); rrEx239[syr-5p::GFP::ATGL-1(S303A)::HA; rol-6(gf)] are described in [9 (link)]. The strain atgl-1(tm3116) harbors a 423bp deletion of the atgl-1 gene and was obtained from National BioResource Project, Tokyo, Japan. Rabbit polyclonal antibody against ATGL-1 was raised using a synthetic peptide CTKRKVPDEPTTSKR (GenScript). Anti-PAR-5 antibody was a gift from Dr. Andy Golden. Anti-GFP (abcam ab290), anti-ubiquitin (Santa Cruz SC8017) and anti-P-14-3-3 (Cell Signaling #2981S) antibodies are available commercially.

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Xie M, & Roy R. (2015). AMP-Activated Kinase Regulates Lipid Droplet Localization and Stability of Adipose Triglyceride Lipase in C. elegans Dauer Larvae. PLoS ONE, 10(6), e0130480.

Publication 2015

CTKRKVPDEPTTSKR ab290 anti-ubiquitin ( SC8017

14 3 3 Anti antibody Antibodies Antibody C elegans Gene deletion Peptide Rabbit Strain Ubiquitin

Corresponding Organization : McGill University

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Variable analysis

independent variables

Strain VS20 hjIs67[Patgl-1::atgl-1::GFP]
Strain CB1370 daf-2(e1370)
Strain MR1000 daf-2(e1370); aak-1(tm1944); aak-2(ok524)
Strain MR1348 daf-2(e1370); rrEx226[sur-5p::GFP::ATGL-1::HA; rol-6(gf)]
Strain MR1413 daf-2(e1370); rrEx239[syr-5p::GFP::ATGL-1(S303A)::HA; rol-6(gf)]
Strain atgl-1(tm3116)

dependent variables

Expression of ATGL-1 protein
Localization of ATGL-1 protein

control variables

Culture conditions for C. elegans strains as previously described by Brenner [11]
Rabbit polyclonal antibody against ATGL-1
Anti-PAR-5 antibody
Anti-GFP antibody
Anti-ubiquitin antibody
Anti-P-14-3-3 antibody

positive controls

Strain VS20 hjIs67[Patgl-1::atgl-1::GFP]

negative controls

Strain atgl-1(tm3116)

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