Contractility Measurement of Rat Jejunum Muscularis

Muscularis externa was dissected from the jejunum of 6–8 weeks old Sprague Dawley rat as previously described.11 (link) Tissues were transduced with non-targeting or let-7f lentiviral particles (Dharmacon) with a multiplicity of infection of 5. At day 0 and day 5, about 2 min long movies were recorded at a rate of 14 frames/second using a DP22 camera (Olympus) connected to a SZ61 stereomicroscope (Olympus) and used for contractility measurement and spatial–temporal mapping.

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Mazzone A., Strege P.R., Gibbons S.J., Alcaino C., Joshi V., Haak A.J., Tschumperlin D.J., Bernard C.E., Cima R.R., Larson D.W., Chua H.K., Graham R.P., El Refaey M., Mohler P.J., Hayashi Y., Ordog T., Calder S., Du P., Farrugia G, & Beyder A. (2019). microRNA overexpression in slow transit constipation leads to reduced NaV1.5 current and altered smooth muscle contractility. Gut, 69(5), 868-876.

Publication 2019

DP22 camera SZ61 stereomicroscope

Contractility Frames Infection Jejunum Muscularis Sprague dawley rat Tissues

Corresponding Organization : Mayo Clinic

Other organizations : WinnMed, The Ohio State University Wexner Medical Center, University of Auckland

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Variable analysis

independent variables

Lentiviral transduction with non-targeting or let-7f particles

dependent variables

Contractility measurement
Spatial–temporal mapping

control variables

Sprague Dawley rat jejunum (6–8 weeks old)
Muscularis externa tissue
Recording parameters (2 min long movies, 14 frames/second)

controls

Negative control: Non-targeting lentiviral particles

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