Measuring Biomarkers in Experimental Models

Kallistatin levels were measured using a human kallistatin ELISA kit (R&D Systems, Minneapolis, MN) and a mouse kallistatin ELISA kit (Fine Biological Technology, Wuhan, China); fibronectin was measured using a mouse fibronectin ELISA kit (Assaypro, St. Charles, MO), ICAM-1 using a mouse ICAM-1 ELISA kit (R&D systems, Minneapolis, MN), Ang II by a ELISA kit (Enzo Life Sciences, Farmingdale, NY), and erythropoietin (EPO) using a mouse EPO ELISA kit (R&D systems, Minneapolis, MN). Western blot analysis was performed as described (30 (link)). The primary antibodies used were purchased from the following companies: mouse kallistatin (SerpinA3C) (R&D Systems, Minneapolis, MN); TGF-β1 (R&D Systems, Minneapolis, MN), TβR-I and TβR-II (Novus Biologicals, Littleton, CO); CTGF (Santa Cruz, Dallas, TX); TNF-α (Cell Signaling Technology, Danvers, MA); 3-NT, NOX4 and NOX2 (Abcam, Cambridge, MA); ACE (R&D systems, Minneapolis, MN); HIF-1α (Novus Biologicals, Littleton, CO) and β-actin (Sigma-Aldrich, St. Louis, MO).

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Yang Y., He X., Cheng R., Chen Q., Shan C., Chen L, & Ma J. (2020). Diabetes‐induced upregulation of kallistatin levels exacerbates diabetic nephropathy via RAS activation. The FASEB Journal, 34(6), 8428-8441.

Publication 2020

human kallistatin ELISA kit mouse fibronectin ELISA kit mouse ICAM-1 ELISA kit ELISA kit mouse EPO ELISA kit mouse kallistatin (SerpinA3C) TGF-β1 TβR-I and TβR-II TNF-α HIF-1α β-actin

Actin Antibodies Biological Biologicals Ctgf Elisa Erythropoietin Fibronectin Human Icam 1 Kallistatin Mouse Novus Nox4 Tgf β1 Tnf α Tβr i Tβr ii Western blot analysis

Corresponding Organization : Tianjin Medical University

Other organizations : Third Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University

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Variable analysis

independent variables

Kallistatin levels measured using human kallistatin ELISA kit
Kallistatin levels measured using mouse kallistatin ELISA kit
Fibronectin measured using mouse fibronectin ELISA kit
ICAM-1 measured using mouse ICAM-1 ELISA kit
Angiotensin II (Ang II) measured using ELISA kit
Erythropoietin (EPO) measured using mouse EPO ELISA kit

dependent variables

Kallistatin levels
Fibronectin levels
ICAM-1 levels
Angiotensin II (Ang II) levels
Erythropoietin (EPO) levels

control variables

Western blot analysis performed as described (30 (link))
Primary antibodies used for Western blot analysis

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