Immunofluorescence Microscopy Antibodies

Rabbit anti-Ror2 ^{61 (link)} and anti-IFT20 ^{31 (link)} antibodies were prepared as described previously. Sheep anti-TGN46 and rabbit anti-Giantin antibodies have been described previously^{62 (link)}. Following antibodies were purchased commercially: mouse anti-GM130 (35, BD), anti-Cortactin (4F11, Millipore), anti-γ-tubulin (GTU-88, Sigma), anti-tyrosinated tubulin (TUB01A2, Sigma), anti-AKAP450 (15, BD), anti-GFP (JL-8, Clontech), anti-acetylated tubulin (6-11B-1, Sigma), anti-Myc (9E10, Santa Cruz), anti-Golgin-97 (CDF4, Thermo), and Alexa Fluor 647-conjugated anti-GM130 (5G8, MBL); rabbit anti-IFT20 (13615-1-AP, Proteintech), anti-Arl13B (ab83879, Abcam), anti-GM130 (PM061, MBL), anti-γ-tubulin (T5192, Sigma), anti-Golgin-97 (D8P2K, Cell Signaling Technology), and HRP-conjugated anti-α-tubulin (PM054-7, MBL).

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Nishita M., Park S.Y., Nishio T., Kamizaki K., Wang Z., Tamada K., Takumi T., Hashimoto R., Otani H., Pazour G.J., Hsu V.W, & Minami Y. (2017). Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Scientific Reports, 7, 1.

Publication 2017

mouse anti-GM130 anti-Cortactin (4F11, anti-γ-tubulin (GTU-88, anti-GFP (JL-8, anti-Myc (9E10, ab83879 anti-Golgin-97 (D8P2K,

Alexa fluor 647 Anti antibodies Antibodies Cortactin Giantin Golgin 97 Mouse Rabbit Ror2 Sheep Tubulin α tubulin

Corresponding Organization : Kobe University

Other organizations : Brigham and Women's Hospital, Harvard University, RIKEN Center for Brain Science, Shimane University, University of Massachusetts Chan Medical School

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Protocol cited in 122 other protocols

Variable analysis

independent variables

Rabbit anti-Ror2 antibody
Anti-IFT20 antibody
Sheep anti-TGN46 antibody
Rabbit anti-Giantin antibody
Mouse anti-GM130 antibody
Anti-Cortactin antibody
Anti-γ-tubulin antibody
Anti-tyrosinated tubulin antibody
Anti-AKAP450 antibody
Anti-GFP antibody
Anti-acetylated tubulin antibody
Anti-Myc antibody
Anti-Golgin-97 antibody
Alexa Fluor 647-conjugated anti-GM130 antibody
Rabbit anti-IFT20 antibody
Anti-Arl13B antibody
Anti-GM130 antibody
Anti-γ-tubulin antibody
Anti-Golgin-97 antibody
HRP-conjugated anti-α-tubulin antibody

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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