After
a 24 h incubation, TRIzol reagent (Invitrogen) was used to extract
mRNA according to the instructions.45 (link),46 (link) The RNA was
reverse-transcribed into cDNA using the Rayscript cDNA Synthesis KIT
(GENEray, GK8030, Shanghai, China) and subsequently employed in RT-PCR
reactions with SYBR Green (GENEray, GK8030, Shanghai, China).Table 4 describes the primers
of cytokines. Reactions were carried out on an ABI7500 apparatus (Applied
Biosystems Inc., USA). Before being compared with the control, the
level of mRNA to be tested was standardized to the level of GAPDH.
Relative game expression levels were quantitated using the ΔΔCt
method.48 (link)
a 24 h incubation, TRIzol reagent (Invitrogen) was used to extract
mRNA according to the instructions.45 (link),46 (link) The RNA was
reverse-transcribed into cDNA using the Rayscript cDNA Synthesis KIT
(GENEray, GK8030, Shanghai, China) and subsequently employed in RT-PCR
reactions with SYBR Green (GENEray, GK8030, Shanghai, China).
of cytokines. Reactions were carried out on an ABI7500 apparatus (Applied
Biosystems Inc., USA). Before being compared with the control, the
level of mRNA to be tested was standardized to the level of GAPDH.
Relative game expression levels were quantitated using the ΔΔCt
method.48 (link)
