Root Exudates Extraction from Diverse Plants

Root exudates of rapid cycling rape (B. napus), perennial ryegrass (Lolium perenne) and tomato (Solanum lycopersicum) were collected by Petri dish cultivation (PDC) and with a hydrophobic trapping system (HTS). Seeds were surface disinfected with 70% ethanol for 10 min, followed by 1% NaOCl for 10 min, and rinsed three times with sdH₂O. Surface-disinfected seeds were placed on Murashige and Skoog (MS) basal medium covered with a layer of autoclaved cellophane and pre-germinated in a growth chamber at 25°C with 14 h photoperiod. Contaminated seeds were discarded.

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Wang Y., Zheng X., Sarenqimuge S, & von Tiedemann A. (2023). The soil bacterial community regulates germination of Plasmodiophora brassicae resting spores rather than root exudates. PLOS Pathogens, 19(3), e1011175.

Publication 2023

Cellophane Dish Ethanol Exudates Lolium Rape Root Seeds Solanum lycopersicum

Corresponding Organization : University of Göttingen

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Variable analysis

independent variables

Cultivation method (Petri dish cultivation and hydrophobic trapping system)

dependent variables

Root exudates of rapid cycling rape (B. napus), perennial ryegrass (Lolium perenne) and tomato (Solanum lycopersicum)

control variables

Surface disinfection of seeds (70% ethanol for 10 min, followed by 1% NaOCl for 10 min, and rinsed three times with sdH2O)
Pre-germination of surface-disinfected seeds on Murashige and Skoog (MS) basal medium covered with a layer of autoclaved cellophane in a growth chamber at 25°C with 14 h photoperiod
Discarding of contaminated seeds

controls

Positive control: Not specified
Negative control: Not specified

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