Forward Genetic Screening for CEC-5 Regulators

Forward genetic screening was conducted as previously described²⁴. Briefly, to identify factors regulating CEC-5, we chemically mutagenized GFP::CEC-5 strain by ethyl methanesulfonate (EMS), followed by a clonal screening. The F2 progeny worms were visualized under fluorescent microscope at adult stage. One mutant that disrupted the nuclear puncta formation of CEC-5 was isolated from one thousand haploid genomes. CEC-5(R124C) was identified by genome re-sequencing.

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Hou X., Xu M., Zhu C., Gao J., Li M., Chen X., Sun C., Nashan B., Zang J., Zhou Y., Guang S, & Feng X. (2023). Systematic characterization of chromodomain proteins reveals an H3K9me1/2 reader regulating aging in C. elegans. Nature Communications, 14, 1254.

Publication 2023

Adult Clonal Ethyl methanesulfonate Genome Microscope Strain Worms

Corresponding Organization : Chinese Academy of Sciences

Other organizations : University of Science and Technology of China

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Variable analysis

independent variables

Chemical mutagenesis by ethyl methanesulfonate (EMS)

dependent variables

Formation of nuclear puncta of CEC-5

control variables

GFP::CEC-5 strain

controls

No positive or negative controls were explicitly mentioned.

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