ADNVs were collected and their genomic DNA was isolated using DNeasy Blood & Tissue Kits (Qiagen, 69504). DNA in the nuclear, chloroplast and mitochondria was identified by PCR (Takara, R010A) following the manufacturer’s instructions. The primer used for target genes were shown in Additional file 1: Table S2. The products were separated by electrophoresis on a 1% agarose gel stained with ethidium bromide (EB).
Free full text: Click here