Cytotoxic Effects of Phytochemicals on Vero-E6 Cells

The cytotoxic effect of selected phytochemicals were carried out with Vero-E6 cells using PrestoBlue™ Cell Viability kit as described earlier (Lewis et al., 2022 (link)). Briefly, Vero-E6 cells (20,000 cells) were seeded overnight in 96 well plates. Then, 100 µL of complete media (EMEM+10% FBS+1%Pen/Strep) was added to refresh the cells along with 50, 100, and 200 µM selected phytochemicals, and incubation was continued for 24, 48, and 72 hs at 37°C respectively. Cytotoxicity detection regent (PrestoBlue solution) was added and incubated for additional 1h at 37°C. The absorbance was taken at 530/25 excitation and 590/35 nm emission using Bio-Tek Synergy HT fluorescent plate reader.