According to the manufacturer’s instructions, serum levels of leptin, adiponectin and resistin were measured using a rat insulin ELISA kit from Ray Biotech (3607 Parkway Lane, IL, USA). <10.0% for the intra-assay coefficient of variance and <12.0% for the inter-assay coefficient of variation. Leptin levels were indicated in pg/mL, whilst adiponectin and resistin results were expressed in ng/mL.
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