Antimicrobial Susceptibility Profiling

Anonymized clinical Staphylococcus aureus PECHA 10, Pseudomonas aeruginosa PECHA 4, and Candida albicans X3 strains (Di Giulio et al., 2018 (link), 2020 (link)) were used for this study. The strains were isolated from chronic wounds of patients that gave their informed consent for the study. The study was approved by the Inter Institutional Ethic Committee of University “G. d’Annunzio” Chieti-Pescara, Chieti, Italy (ID n. richycnvw). The strains were characterized for their susceptibility to antibiotics, and in particular, S. aureus PECHA 10 and P. aeruginosa PECHA 4 were resistant strains (Supplementary Table 1). All the methods were performed in accordance with the relevant guidelines and regulations. For the experiments, bacteria were cultured in Trypticase Soy Broth (TSB, Oxoid, Milan, Italy) and incubated at 37°C overnight in aerobic condition and then refreshed for 2 h at 37°C in an orbital shaker. Then the broth cultures were standardized to optical density at 600 nm (OD₆₀₀) = 0.125. The broth culture of Candida albicans, grown on Sabouraud dextrose agar (SAB, Oxoid, Milan, Italy) was prepared in RPMI 1640 (Sigma-Aldrich, Milan, Italy) plus 2% glucose and standardized to OD₆₀₀ = 0.15.