SERS has been induced by using home-made Gold nanoparticles (GNP) onto conventional microscope slides. A colloid of GNPs has been obtained by a conventional citrate reduction method [15 (link),16 (link)]. A HAuCl solution was reduced by sodium citrate with vigorous stirring at near boiling temperature. The amount of sodium citrate was defined in order to obtain GNPs with controlled size with particle diameter in the range of 20–50 nm. Preliminary tests have shown that the GNPs with a diameter equal to ∼27 nm give optimum SERS features [16 (link)].
Particle size, preparation stability and Raman signal of the resulting preparations have been investigated by means of absorption spectroscopy, Transmission Electron Microscop (TEM), and Dynamic Light Scattering (DLS). In particular, absorption spectroscopy was used to get information on the plasmon resonance peak and to study the stability of the preparations [17 (link),18 (link)]. TEM and DLS were employed to estimate the size of the GNPs. Absorption spectra of the prepared GNPs were recorded by UV–VIS spectroscopy by a two-beams spectrophotometer (LS25, Perkin Elmer, Waltham, MA, USA).
Hydrogen tetrachloroaurate (HAuCl ), and trisodium citrate were purchased from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). All chemicals were used as received. Rhodamine 6G were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Particle size, preparation stability and Raman signal of the resulting preparations have been investigated by means of absorption spectroscopy, Transmission Electron Microscop (TEM), and Dynamic Light Scattering (DLS). In particular, absorption spectroscopy was used to get information on the plasmon resonance peak and to study the stability of the preparations [17 (link),18 (link)]. TEM and DLS were employed to estimate the size of the GNPs. Absorption spectra of the prepared GNPs were recorded by UV–VIS spectroscopy by a two-beams spectrophotometer (LS25, Perkin Elmer, Waltham, MA, USA).
Hydrogen tetrachloroaurate (HAuCl ), and trisodium citrate were purchased from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). All chemicals were used as received. Rhodamine 6G were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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