Bt OMVs were observed using negative staining with transmission electron microscopy (TEM) as previously described [53 (link)]. Briefly, isolated Bt OMVs were adsorbed to carbon–formvar-coated copper EM grids (Agar Scientific) for 1 min before wicking off with filter paper and negatively staining with 2% uranyl acetate solution (BDH) in water for 1 min. Grids were air-dried before analysis using a Tecnai G2 20 Twin TEM (FEI) at ×29,000 magnification.
Corresponding Organization : Imperial College London
Other organizations :
Quadram Institute, Nottingham Trent University, University of Reading, London North West Healthcare NHS Trust, University of East Anglia
Observation of Bt OMVs using negative staining with transmission electron microscopy (TEM)
control variables
Carbon–formvar-coated copper EM grids
2% uranyl acetate solution in water for 1 min negative staining
Tecnai G2 20 Twin TEM at ×29,000 magnification
controls
No positive or negative controls were explicitly mentioned in the provided text.
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