Quantifying Cholera Toxin in OMVs
Corresponding Organization : BioTechMed-Graz
Variable analysis
- Different concentrations of purified CT with known concentrations
- Concentration of CT in OMV and cell-free supernatant samples
- Equal volumes of supernatant and equal volumes of OMV samples were serially diluted
- GM1 ganglioside coated plates were used
- 1% (wt/vol) fatty acid-free bovine serum albumin (BSA) was used to block the GM1-coated plates
- Rabbit anti-CT polyclonal antibody (1:10,000) and HRP-linked goat ani-rabbit IgG antibody (1:2,000) were used
- Tetramethylbenzidine (TMB) substrate solution was used for color development
- Purified CT with known concentrations were used as the standards
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