Gill Protein Extraction and ATPase Assays

The total protein of each gill sample was extracted using a Protein Extraction Kit (Invent, USA), and the protein concentration was then determined using a BCA Protein Assay Kit (Merck, GER) [7 (link)]. Subsequently, NKA activity was measured using a Na⁺-K⁺-ATPase Kit (Jiancheng, CHN) according to the manufacturer’s instructions [7 (link)], and H⁺-K⁺-ATPase activity was measured following the protocol supplied by the manufacturer of the H⁺-K⁺-ATPase Kit (JianCheng, CHN). ATP content assays were performed using an ATP assay kit (Jiancheng, CHN), following the manufacturer’s instructions.

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Su M., Liu N., Zhang Z, & Zhang J. (2022). Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes. BMC Genomics, 23, 545.

Publication 2022

Protein Extraction Kit BCA Protein Assay Kit Na+-K+-ATPase Kit ATP assay kit

A protein Assay Gill H k atpase Na k atpase Protein

Corresponding Organization :

Other organizations : Shenzhen University

Top 5 similar protocols

Variable analysis

independent variables

Protein extraction method (Protein Extraction Kit)
Protein concentration measurement method (BCA Protein Assay Kit)
NKA activity measurement method (Na+-K+-ATPase Kit)
H+-K+-ATPase activity measurement method (H+-K+-ATPase Kit)
ATP content measurement method (ATP assay kit)

dependent variables

Protein concentration
NKA activity
H+-K+-ATPase activity
ATP content

control variables

Gill samples

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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