Characterization and Validation of BPLER and HMLER Cell Lines

The BPLER and HMLER cells were established previously^{20 (link)} and characterized extensively^{4 (link),20 (link),90 (link)–98 (link)}. The BPLER cells are cultured in the BMI-T medium (US Biological, cat# 506387.500, or TumoriGenesis Product No: 833)^{5 (link)}, and the HMLER cells are cultured in MEGM medium (Lonza,cat# CC-3150)^{4 (link),20 (link)}. The BPLER and HMLER cells we established were tested for mycoplasma and deposited to ATCC (American Type Culture Collection, ATCC item #s CRL-3546 and CRL-3547) and European Collection of Authenticated Cell Cultures (ECACC, Accession numbers; 20012030, 20012033, 20012038, 20012041, 20012044, and 20012047, https://www.ukbrcn.org/news/new-accessions-coming-soon-to-the-european-collection-of-authenticated-cell-cultures/).

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Kamran M., Bhattacharya U., Omar M., Marchionni L, & Ince T.A. (2022). ZNF92, an unexplored transcription factor with remarkably distinct breast cancer over-expression associated with prognosis and cell-of-origin. NPJ Breast Cancer, 8, 99.

Publication 2022

MEGM medium

Biological Cell cultures Cells Cultured medium European Mycoplasma Tumorigenesis

Corresponding Organization :

Other organizations : Cornell University

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Variable analysis

independent variables

Cell line (BPLER or HMLER)

dependent variables

Not explicitly mentioned

control variables

Mycoplasma testing
Cell line deposition to ATCC and ECACC

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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