The production of TK-LP@(GP + EM) NPs was carried out using the thin film hydration method, as previously reported [30 (link)]. In this process, 20 mg of soybean lecithin, 10 mg of cholesterol, and 6 mg of DSPE-TK-PEG2000 were dissolved in 5 mL of CHCl3. A solution of 1 mg/mL Emodin was added to this mixture. The solution was then transferred to a round-bottomed flask, and the organic solvent was evaporated using a rotary evaporator, forming a lipid film at the bottom of the flask.
Next, 3 mL of a PBS solution containing 2% Tween-80, 0.5% MethylCellulose and Geniposide (3 mg) and was added to the flask to hydrate the lipid film. The mixture was sonicated on ice for 10 min. Subsequently, the emulsion nanoliposome suspension was obtained by passing it through a 0.45 μm microporous filtration membrane, effectively removing free Emodin and Geniposide through centrifugation at 13,000 rpm for 30 min.
The resulting TK-LP@(GP + EM) NPs were then mixed with the Møm solution according to the ratio of lecithin (Møm: liposome = 1:10(w/w)), and the mixture was stirred at 37 °C for 2 h. The uniform TK-MLP@(GP + EM) NPs were obtained by sonication in a water bath and extrusion through a 0.22 μm polyethersulfone membrane.
Next, 3 mL of a PBS solution containing 2% Tween-80, 0.5% MethylCellulose and Geniposide (3 mg) and was added to the flask to hydrate the lipid film. The mixture was sonicated on ice for 10 min. Subsequently, the emulsion nanoliposome suspension was obtained by passing it through a 0.45 μm microporous filtration membrane, effectively removing free Emodin and Geniposide through centrifugation at 13,000 rpm for 30 min.
The resulting TK-LP@(GP + EM) NPs were then mixed with the Møm solution according to the ratio of lecithin (Møm: liposome = 1:10(w/w)), and the mixture was stirred at 37 °C for 2 h. The uniform TK-MLP@(GP + EM) NPs were obtained by sonication in a water bath and extrusion through a 0.22 μm polyethersulfone membrane.
Free full text: Click here
