Antibodies for BMPR1B (ab155058) and GAPDH (ab9482) were obtained from Abcam (UK). Western blotting was performed according to a previously reported method (Liu et al., 2016 (link)).
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Protocol detail
Western Blot Analysis of BMPR1B and GAPDH
Corresponding Organization :
Other organizations : Animal Science Research Institute, Beijing Institute of Optoelectronic Technology, Fifth Affiliated Hospital of Xinjiang Medical University, Xinjiang Medical University, Xinjiang Uygur Autonomous Region Disease Prevention and Control Center
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Variable analysis
independent variables
- Antibodies for BMPR1B (ab155058) and GAPDH (ab9482) obtained from Abcam (UK)
- Protein expression levels of BMPR1B and GAPDH
- Previously reported method (Liu et al., 2016) used for Western blotting
- No positive or negative controls were explicitly mentioned in the input.
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