Doxycycline-Inducible Transgene Expression
Corresponding Organization : Tianjin Medical University General Hospital
Other organizations : Peking University, Center for Life Sciences
Variable analysis
- Plasmid (Addgene #50917) containing the fragment of dCas9-KRAB
- Successful introduction of the dCas9-KRAB fragment into the Gateway Entry vector pCR8/GW/TOPO
- Successful subcloning of the right donor into the destination vector p2Lox-FLAG
- Manufacturer's protocol for introducing the fragment into the Gateway Entry vector
- Gateway Technology (Invitrogen) for subcloning the right donor into the destination vector
- None specified
- None specified
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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