The fragment of dCas9-KRAB was amplified from the plasmid (Addgene #50917) and introduced into Gateway Entry vector pCR8/GW/TOPO (Invitrogen, United States) following the manufacturer’s protocol and verified by sequencing. The right donor was subcloned into the destination vector p2Lox-FLAG [18 (link)] by Gateway Technology (Invitrogen).
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