Protocol detail

Transient transfections of BRG1 in HepAD38 cells

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Transient transfections in HepAD38 cells were performed employing Lipofectamine 2000 (Invitrogen, Grand Island, NY) as described by manufacturer with 10µg of BRG1 mammalian expression plasmid^{8 (link)} (Addgene, Cambridge, MA). BRG1 siRNA and scrambled control siRNA (Invitrogen, Grand Island, NY) were transfected using Lipofectamine RNAi MAX (Invitrogen, Grand Island, NY).

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Fan H., Cui Z., Zhang H., Kailasam Mani S.K., Diab A., Lefrancois L., Fares N., Merle P, & Andrisani O. (2016). DNA demethylation induces SALL4 gene re-expression in subgroups of hepatocellular carcinoma associated with Hepatitis B or C virus infection. Oncogene, 36(17), 2435-2445.

Publication 2016

Lipofectamine 2000 BRG1 mammalian expression plasmid8 scrambled control siRNA Lipofectamine RNAi MAX

Brg1 Cells Lipofectamine Lipofectamine 2000 Mammalian Rnai Sirna Transfections Transient

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Variable analysis

independent variables

BRG1 mammalian expression plasmid
BRG1 siRNA
Scrambled control siRNA

dependent variables

Not explicitly mentioned

control variables

Lipofectamine 2000 (transfection agent)
Lipofectamine RNAi MAX (transfection agent)
HepAD38 cell line

controls

Positive control: BRG1 mammalian expression plasmid
Negative control: Scrambled control siRNA

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