Aging and Stress Response in C. elegans

At the stage of 1 day of adulthood, synchronized gst-4::GFP, sod-3::GFP, daf-16::GFP and skn-1::GFP transgenic worms fed heat killed OP50 and 10 mM 2-HIBA from embryo hatching were anesthetized with sodium azide (20 mmol L⁻¹) (Sigma-Aldrich, St. Louis, MO, United States) and observed by Zeiss Axiovert 25 microscope as described in (Bianchi et al., 2020 (link)). The experiments were repeated three times and 15 worms per group were used in each experiment. Images were taken at the time of exposure of 0.2 s and fluorescence was analyzed using ImageJ software. Scale bars were inserted by Zeiss ZEN Microscopy Software 2011.

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Schifano E., Conta G., Preziosi A., Ferrante C., Batignani G., Mancini P., Tomassini A., Sciubba F., Scopigno T., Uccelletti D, & Miccheli A. (2022). 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in Caenorhabditis elegans. Frontiers in Molecular Biosciences, 9, 986022.

Publication 2022

sodium azide Axiovert 25 microscope ZEN Microscopy Software 2011

Embryo Fluorescence Microscopy Sodium azide Transgenic Worms

Corresponding Organization : Sapienza University of Rome

Other organizations : Italian Institute of Technology

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Variable analysis

independent variables

Feeding of heat killed OP50
Feeding of 10 mM 2-HIBA from embryo hatching

dependent variables

Expression of gst-4::GFP
Expression of sod-3::GFP
Expression of daf-16::GFP
Expression of skn-1::GFP

control variables

Synchronized transgenic worms at 1 day of adulthood
Anesthetization with sodium azide (20 mmol L^-1)
Observation by Zeiss Axiovert 25 microscope
Exposure time of 0.2 s
Fluorescence analysis using ImageJ software
Scale bars inserted by Zeiss ZEN Microscopy Software 2011

controls

No positive or negative controls were explicitly mentioned.

Annotations

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