Ultrastructural Analysis of Optic Nerve

After the immersion fixation, the optic nerves from each animal (n = 7–9) were incubated at 4 °C until further washing with 90 mM Na-cacodylate buffer with subsequent processing as described previously [20 (link)]. Electron micrographs for analysis were collected using an Orius SC200B CCD camera (Gatan Inc., Warrendale, PA, USA) mounted on a of Tecnai G² Spirit TWIN/BioTWIN transmission electron microscope (FEI Europe B.V, Eindhoven, The Netherlands). Representative figures for illustrations were collected using a side mounted Veleta CCD camera (Olympus).

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Jagomäe T., Seppa K., Reimets R., Pastak M., Plaas M., Hickey M.A., Kukker K.G., Moons L., De Groef L., Vasar E., Kaasik A., Terasmaa A, & Plaas M. (2021). Early Intervention and Lifelong Treatment with GLP1 Receptor Agonist Liraglutide in a Wolfram Syndrome Rat Model with an Emphasis on Visual Neurodegeneration, Sensorineural Hearing Loss and Diabetic Phenotype. Cells, 10(11), 3193.

Publication 2021

Orius SC200B CCD camera Veleta CCD camera

Animal Buffer Cacodylate Electron Immersion Optic nerves Transmission electron microscope Twin

Corresponding Organization : University of Tartu

Other organizations : Tartu University Hospital, KU Leuven

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Variable analysis

independent variables

Immersion fixation
Incubation of optic nerves at 4 °C

dependent variables

Electron micrographs for analysis

control variables

Washing with 90 mM Na-cacodylate buffer
Processing as described previously [20 (link)]

controls

Positive control: Not specified
Negative control: Not specified

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