Immunohistochemistry of Receptor Markers

Immunohistochemistry (IHC) was performed as previously described (23 (link)). Antibodies used were as follows: AR (AR441, 1:500, DAKO), ERα (SP1, 1:100, Thermo-Fisher), and PR (1294, 1:500, DAKO). Images were captured using the Aperio Digital Pathology system (Leica Biosystems), assembled in Adobe Photoshop CC, and percent positive immunoreactivity determined for triplicate samples using Imagescope software (Leica).

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Finlay-Schultz J., Gillen A.E., Brechbuhl H.M., Ivie J.J., Matthews S.B., Jacobsen B.M., Bentley D.L., Kabos P, & Sartorius C.A. (2017). Breast cancer suppression by progesterone receptors is mediated by their modulation of estrogen receptors and RNA polymerase III. Cancer research, 77(18), 4934-4946.

Publication 2017

AR441 Aperio Digital Pathology system Imagescope software

Antibodies Immunohistochemistry

Corresponding Organization : University of Colorado Anschutz Medical Campus

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Variable analysis

independent variables

Antibodies used: AR (AR441, 1:500, DAKO), ERα (SP1, 1:100, Thermo-Fisher), and PR (1294, 1:500, DAKO)

dependent variables

Percent positive immunoreactivity

control variables

Immunohistochemistry (IHC) protocol as previously described (23 (link))
Image capture using the Aperio Digital Pathology system (Leica Biosystems)
Image assembly in Adobe Photoshop CC
Percent positive immunoreactivity determination using Imagescope software (Leica)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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