Purification and Characterization of Mannose Biosynthetic Enzymes

DEAE-Sepharose ff and Superdex-75 were purchased from GE Healthcare Life Sciences, Milan, Italy. Phosphoglucose isomerase from rabbit muscle, phosphomannose isomerase from E. coli, glucose 6-phosphate dehydrogenase from baker’s yeast (S. cerevisiae), phosphoglucomutase from rabbit muscle, α-d(+)Mannose 1-phosphate sodium salt hydrate, and β-Nicotinamide adenine dinucleotide phosphate sodium salt, were purchased from Sigma-Aldrich, Milan, Italy. Sypro Orange was from Invitrogen Molecular Probes, Monza, Italy. Mannose-1,6-bisphosphate was synthesized and purified, as described [3 (link)]. All of the other reagents were of analytical grade.

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Citro V., Cimmaruta C., Monticelli M., Riccio G., Hay Mele B., Cubellis M.V, & Andreotti G. (2018). The Analysis of Variants in the General Population Reveals That PMM2 Is Extremely Tolerant to Missense Mutations and That Diagnosis of PMM2-CDG Can Benefit from the Identification of Modifiers. International Journal of Molecular Sciences, 19(8), 2218.

Publication 2018

DEAE-Sepharose ff Superdex-75 Phosphoglucose isomerase from rabbit muscle phosphomannose isomerase from E. coli glucose 6-phosphate dehydrogenase from baker’s yeast phosphoglucomutase from rabbit muscle α-d(+)Mannose 1-phosphate sodium salt hydrate β-Nicotinamide adenine dinucleotide phosphate sodium salt Sypro Orange

Deae E coli Glucose 6 phosphate dehydrogenase Mannose Mannose 1 phosphate Molecular probes Muscle Nicotinamide adenine dinucleotide phosphate Phosphoglucomutase Phosphoglucose isomerase Phosphomannose isomerase Rabbit S cerevisiae Salt Sepharose Sodium Sodium hydrate

Corresponding Organization : University of Naples Federico II

Other organizations : Istituto di Chimica Biomolecolare

Top 5 similar protocols

Variable analysis

independent variables

DEAE-Sepharose ff
Superdex-75

dependent variables

Not explicitly mentioned

control variables

Phosphoglucose isomerase from rabbit muscle
Phosphomannose isomerase from E. coli
Glucose 6-phosphate dehydrogenase from baker's yeast (S. cerevisiae)
Phosphoglucomutase from rabbit muscle
α-D(+)Mannose 1-phosphate sodium salt hydrate
β-Nicotinamide adenine dinucleotide phosphate sodium salt
Sypro Orange
Mannose-1,6-bisphosphate
All other reagents were of analytical grade

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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