Mucosal and Systemic Ig Quantification

Eight week-old female C57BL/6J mice were purchased from The Jackson Laboratory in Bar Harbor (Maine, USA). Four animals were housed in each cage in a temperature- and humidity-controlled room with alternating light/dark cycles of 12 h, with access to Laboratory Autoclavable Rodent Diet 5010 (LabDiet, St. Louis, MO) and water ad libitum. All experimental procedures were approved by the University of Louisville's Institutional Animal Care and Use Committee. Mice were acclimated for approximately one week prior to the initiation of the studies. Mouse immunization, sample collection and quantification of mucosal and systemic Igs were performed as previously described26 (link).